The Giants started the season 0-2 for the second straight season with a 20-13 loss to the Dallas Cowboys on Sunday at AT&T Stadium.
New York was outgained 299-254 as Odell Beckham Jr. was held to four catches for 50 yards and the team was limited to just 35 yards on the ground.
Eli Manning was sacked six times for 59 yards and lost a fumble, while Cowboys quarterback Dak Prescott threw for 160 yards and a touchdown and ran for 46 yards.
Rookie running back Saquon Barkley, who had 106 rushing yards and a 68-yard touchdown in his NFL debut last Sunday against the Jacksonville Jaguars, had 11 carries for 28 yards and 14 catches for 80 yards, finishing as the Giants' leading rusher and receiver.
Evan Engram totaled seven catches for 67 yards, while Manning threw for 278 yards on 33-of-44 passing.
The Giants found themselves trailing early on when Prescott threw a 64-yard touchdown to Tavon Austin with 13:37 remaining in the first quarter and allowed a field goal on the next drive. New York punted on all five of its first-half possessions, and Manning lost a fumble on its first possession of the second half that helped set up a Cowboys field goal that put the Giants down 13-0.
After Aldrick Rosas' 28-yard field goal with 4:34 remaining in the third put the Giants on the board, the Cowboys later used a 14-play, 82-yard drive that lasted 8 minutes, 23 seconds, capped by an Ezekial Elliott 6-yard touchdown run, to take a 20-3 lead with 5:45 remaining in the fourth.
Engram's 18-yard touchdown with 1:27 remaining made it 20-10, and after the Giants recovered an onside kick, needed a 38-yard field goal by Rosas to get within a touchdown.